Anticorps Polyclonal de lapin anti-ANGPTL2

• Phare
• Validé par KD/KO

ANGPTL2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 12316-1-AP

Synonymes

angiopoietin like 2, Angiopoietin like protein 2, Angiopoietin related protein 2, ANGPTL2, ARP2, HARP

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Galerie de données de validation

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  WB analysis of mouse small intestine using 12316-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12316-1-AP

  mouse heart tissue was subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 12316-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 12316-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12316-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12316-1-AP

  IP result of anti-ANGPTL2 (IP:12316-1-AP, 4ug; Detection:12316-1-AP 1:500) with mouse testis tissue lysate 4800ug.

• 

  IHC staining of human colon cancer using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules 3T3-L1, tissu d'intestin grêle de souris, tissu splénique de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cardiaque humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

12316-1-AP cible ANGPTL2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANGPTL2 Protéine recombinante Ag2969
• Nom complet: angiopoietin-like 2
• Masse moléculaire calculée: 493 aa, 57 kDa
• Poids moléculaire observé: 57-64 kDa
• Numéro d’acquisition GenBank: BC012368
• Symbole du gène: ANGPTL2
• Identification du gène (NCBI): 23452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANGPTL2 (angiopoietin-like 2), a member of the Angptl protein family, is predominantly secreted from adipose tissue and the heart. ANGPTL2 consists of an N-terminus with a conserved coiled-coil domain, two glycosylation sites and a C-terminus with a conserved fibrinogen-like domain. It is a key adipocyte-derived inflammatory mediator that links obesity to systemic INS resistance. ANGPTL2 may play an important role in the pathogenesis of diabetic glomerulopathy.

Publications

Species	Application	Title
rat	WB	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
human	IHC	World J Gastroenterol; Leukocyte immunoglobulin-like receptor B2 overexpression as a promising therapeutic target and noninvasive screening biomarker for colorectal cancer; Authors - Qian-Qian Wang
human	IHC	Biomolecules; Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods; Authors - Haofuzi Zhang
rat	WB	Sci Rep; PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.; Authors - Jing Li
human,mouse	IHC,WB	J Periodontol; Angiopoietin-like protein 2 deficiency promotes periodontal inflammation and alveolar bone loss.; Authors - Chenxi Jiang
human	WB	PLoS One; Angiopoietin-like protein 2 regulates Porphyromonas gingivalis lipopolysaccharide-induced inflammatory response in human gingival epithelial cells.; Authors - Tasuku Ohno ; KD Validated