Anticorps Polyclonal de lapin anti-AMZ2

AMZ2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 16664-1-AP

Synonymes

AMZ2, Archaemetzincin 2

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 16664-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16664-1-AP (AMZ2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16664-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16664-1-AP (AMZ2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16664-1-AP (AMZ2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16664-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, tissu cérébral humain
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16664-1-AP cible AMZ2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMZ2 Protéine recombinante Ag10141
• Nom complet: archaelysin family metallopeptidase 2
• Masse moléculaire calculée: 360 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC056271
• Symbole du gène: AMZ2
• Identification du gène (NCBI): 51321
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMZ2(archaelysin family metallopeptidase 2) is a zinc metalloprotease. Its calculated molecular weight and observed molecular weight are both 41kDa.