Anticorps Monoclonal anti-AMPD2

AMPD2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF

Conjugaison

Non conjugué

CloneNo.

2C4C1

N° de cat : 67430-1-Ig

Synonymes

AMP deaminase 2, AMP deaminase isoform L, AMPD2

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Galerie de données de validation

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  WB analysis using 67430-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67430-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67430-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 67430-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67430-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67430-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67430-1-Ig (AMPD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67430-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67430-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67430-1-Ig (AMPD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67430-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMPD2 antibody (67430-1-Ig, Clone: 2C4C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules 4T1, cellules HeLa, cellules HepG2, cellules L02, cellules Neuro-2a
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:20000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67430-1-Ig cible AMPD2 dans les applications de WB, IHC, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AMPD2 Protéine recombinante Ag8433
• Nom complet: adenosine monophosphate deaminase 2 (isoform L)
• Masse moléculaire calculée: 879aa,101 kDa; 798aa,92 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: BC007711
• Symbole du gène: AMPD2
• Identification du gène (NCBI): 271
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adenosine monophosphate deaminase (AMPD) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle. Three AMPDs are present in mammals named AMPD1/2/3. AMPD2 is the main activity present in adult human liver and is widely expressed in non-muscle tissues and cells (PMID: 8526848). AMPD2 has some isoforms with the molecular mass of 88-101 kDa.