Anticorps Polyclonal de lapin anti-AMPD1-Specific

• Phare
• Validé par KD/KO

AMPD1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 19780-1-AP

Synonymes

AMP deaminase 1, AMP deaminase isoform M, AMPD1, AMPD1-Specific, MAD, MADA, Myoadenylate deaminase

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 19780-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 19780-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 19780-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300.

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  IHC staining of mouse skeletal muscle using 19780-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 19780-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris,
• Résultats positifs en IHC: tissu de muscle squelettique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

19780-1-AP cible AMPD1-Specific dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: adenosine monophosphate deaminase 1 (isoform M)
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 75-83 kDa
• Numéro d’acquisition GenBank: NM_000036
• Symbole du gène: AMPD1
• Identification du gène (NCBI): 270
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMPD1, also named as MAD and MADA, belongs to the adenosine and AMP deaminases family. AMP deaminase plays a critical role in energy metabolism. It catalyzes the reaction: AMP + H2O = IMP + NH3. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM) which is a metabolic disorder resulting in exercise-related myopathy. The antibody is specific to AMPD1.

Publications

Species	Application	Title
mouse	WB	iScience; Phosphate depletion in insulin-insensitive skeletal muscle drives AMPD activation and sarcopenia in chronic kidney disease; Authors - Ana Andres-Hernando ; KO Validated
human	IHC	Front Oncol; AMPD1 Is Associated With the Immune Response and Serves as a Prognostic Marker in HER2-Positive Breast Cancer.; Authors - Long Wang
mouse	WB	Sci Adv; Lactate metabolism is essential in early-onset mitochondrial myopathy; Authors - Zhenkang Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Laila (Verified Customer) (07-22-2022)

This antibody worked great with Bovine tissue with a total of 60 ug of protein and very little non-specific binding.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: bovine skeletal muscle

AMPD1-Specific Antibody Western Blot validation (1:1000 dilution) in bovine skeletal muscle (Cat no:19780-1-AP)