Anticorps Polyclonal de lapin anti-AMOTL2

• Phare
• Validé par KD/KO

AMOTL2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (2)

N° de cat : 23351-1-AP

Synonymes

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

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Galerie de données de validation

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  WB analysis using 23351-1-AP

  HEK-293 cells lysates were subjected to SDS PAGE followed by western blot with 23351-1-AP (AMOTL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

23351-1-AP cible AMOTL2 dans les applications de WB, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMOTL2 Protéine recombinante Ag19953
• Nom complet: angiomotin like 2
• Masse moléculaire calculée: 779 aa, 86 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC011454
• Symbole du gène: AMOTL2
• Identification du gène (NCBI): 51421
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta. Human AmotL2 encodes two isoforms of a molecular mass of 100 kDa and 60 kDa.(PMID: 34036399,PMID: 25080976)

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Hsa_circ_0024093 accelerates VSMC proliferation via miR-4677-3p/miR-889-3p/USP9X/YAP1 axis in in vitro model of lower extremity ASO.; Authors - Xue Zhang
human	WB	Mol Ther Nucleic Acids; Hypoxic Tumor-Derived Exosomal Long Noncoding RNA UCA1 Promotes Angiogenesis via miR-96-5p/AMOTL2 in Pancreatic Cancer.; Authors - Zengya Guo ; KD Validated
human	WB	Arthritis Res Ther; Regulation of type I interferon signature by VGLL3 in the fibroblast-like synoviocytes of rheumatoid arthritis patients via targeting the Hippo pathway; Authors - Yu Du
human	WB	Sci Rep; MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis.; Authors - Diala Kantar ; KD Validated
human	WB	Mol Biol Cell; AMOT130 drives BMP-SMAD signaling at the apical membrane in polarized cells.; Authors - Patrizia Brunner ; KD Validated
human	WB	Pharmaceuticals (Basel); Comparative Assessment and High-Throughput Drug-Combination Profiling of TEAD-Palmitoylation Inhibitors in Hippo Pathway Deficient Mesothelioma; Authors - Lale Evsen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Florian (Verified Customer) (10-13-2021)

10% SDS-PAGE 1st AB incubation ON

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human breast cancer cell line (HCC1806)

AMOTL2 Antibody Western Blot validation (1:500 dilution) in Human breast cancer cell line (HCC1806) (Cat no:23351-1-AP)

FH

Lisa (Verified Customer) (07-08-2020)

Two specific bands (60 and 100 kDa) and two others non specific.This antibody is working by WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1/600
• Cell Tissue Type: MCF7 cell line

AMOTL2 Antibody Western Blot validation (1/600 dilution) in MCF7 cell line (Cat no:23351-1-AP)