Anticorps Polyclonal de lapin anti-AMOT

• Phare
• Validé par KD/KO

AMOT Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 16870-1-AP

Synonymes

AMOT, angiomotin, KIAA1071

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Galerie de données de validation

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  WB analysis of HEK-293 using 16870-1-AP

  WB result of AMOT antibody (16870-1-AP, 1:600) with si-Control and si-AMOT transfected HEK293 cells.

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  WB analysis of human stomach using 16870-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16870-1-AP (AMOT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 16870-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16870-1-AP (AMOT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu d'estomac humain, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16870-1-AP cible AMOT dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: angiomotin
• Masse moléculaire calculée: 118 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_001113490
• Symbole du gène: AMOT
• Identification du gène (NCBI): 154796
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiomotin belongs to the motin family of angiostatin binding protein. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Most abundant expression was found in placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). This antibody recognizes p130 only.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Hsa_circ_0024093 accelerates VSMC proliferation via miR-4677-3p/miR-889-3p/USP9X/YAP1 axis in in vitro model of lower extremity ASO.; Authors - Xue Zhang
mouse	WB,IF	Int J Cardiol; Conditional knockout of TFPI-1 in VSMCs of mice accelerates atherosclerosis by enhancing AMOT/YAP pathway.; Authors - Jiajun Xiao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jonasz J. (Verified Customer) (05-03-2024)

Very good and specific antibody, detecting a single band at the expected molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells

AMOT Antibody Western Blot validation (1:1000 dilution) in HEK293T cells (Cat no:16870-1-AP)