Anticorps Polyclonal de lapin anti-AMIGO3

AMIGO3 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 21278-1-AP

Synonymes

ALI3, Alivin 3, AMIGO 3, AMIGO3, Amphoterin induced protein 3, KIAA1851

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Galerie de données de validation

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  WB analysis of MCF-7 using 21278-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21278-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21278-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21278-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21278-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 21278-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21278-1-AP (AMIGO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 21278-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21278-1-AP (AMIGO3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HeLa, cellules L02, tissu cérébral humain, tissu ovarien de souris, tissu rénal de souris
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21278-1-AP cible AMIGO3 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMIGO3 Protéine recombinante Ag15790
• Nom complet: adhesion molecule with Ig-like domain 3
• Masse moléculaire calculée: 504 aa, 55 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC110418
• Symbole du gène: AMIGO3
• Identification du gène (NCBI): 386724
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.