Anticorps Polyclonal de lapin anti-ALY

• Phare
• Validé par KD/KO

ALY Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 16690-1-AP

Synonymes

Ally of AML 1 and LEF 1, ALY, ALYREF, BEF, bZIP enhancing factor BEF, THO complex 4, THO complex subunit 4, Tho4, THOC4

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Galerie de données de validation

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  WB analysis of HeLa using 16690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16690-1-AP

  WB result of ALY antibody (16690-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALY transfected HeLa cells.

• 

  WB analysis of HEK-293 using 16690-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16690-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16690-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16690-1-AP (ALY antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16690-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16690-1-AP (ALY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16690-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ALY antibody (16690-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

16690-1-AP cible ALY dans les applications de WB, IHC, IF/ICC, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALY Protéine recombinante Ag10044
• Nom complet: THO complex 4
• Masse moléculaire calculée: 257 aa, 27 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC052302
• Symbole du gène: ALY
• Identification du gène (NCBI): 10189
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
pig	WB	ACS Infect Dis; Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.; Authors - Lin Yang ; KD Validated
human	WB,IF	PLoS Pathog; TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus; Authors - Lingcai Zhao ; KD Validated
human	WB	Nature; mRNA recognition and packaging by the human transcription-export complex; Authors - Belén Pacheco-Fiallos
human	WB	Cell Death Dis; Epigenetic addition of m5C to HBV transcripts promotes viral replication and evasion of innate antiviral responses; Authors - Shuang Ding
human	RIP	Phytomedicine; Niujiao Dihuang Jiedu decoction promotes SLC7A11 m5C methylation modification against ferroptosis in acute-on-chronic liver failure; Authors - Liqiao Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Weak band on WB from HeLa cells extracts but specific for ALYREF/THOC4 (1/1000 dilution, Overnight incubation at 4°C)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa