Anticorps Polyclonal de lapin anti-ALPL

ALPL Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), chIP, ELISA, IF

Conjugaison

Non conjugué

Publications

61

Avis (5)

N° de cat : 11187-1-AP

Synonymes

Alkaline phosphatase, AKP2, AP, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase tissue nonspecific isozyme

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Galerie de données de validation

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  WB analysis using 11187-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11187-1-AP (ALPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11187-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11187-1-AP (ALPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11187-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11187-1-AP (ALPL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 11187-1-AP

  Immunofluorescent analysis of HEK-293 cells using 11187-1-AP (ALPL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11187-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human ALPL (11187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 38 publications below
• IHC: See 19 publications below
• IF: See 5 publications below
• ChIP: See 1 publications below

Informations sur le produit

11187-1-AP cible ALPL dans les applications de WB, IHC, FC (Intra), chIP, ELISA, IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALPL Protéine recombinante Ag1664
• Nom complet: alkaline phosphatase, liver/bone/kidney
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC021289
• Symbole du gène: ALPL
• Identification du gène (NCBI): 249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).This antibody can bind the four mentioned alkaline phosphatases.

Publications

Species	Application	Title
	WB	Mol Ther Nucleic Acids; Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells.; Authors - Juhee Ryu
human	WB	Virol Sin; Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.; Authors - Yingying Song
	IHC	J Cell Physiol; Cell dynamics in Hertwig's epithelial root sheath are regulated by β-catenin activity during tooth root development.; Authors - Siqin Yang
	IHC	Aging (Albany NY); PMID: 32045366; Authors - See-Hyoung Park
human	WB	ACS Biomater Sci Eng; Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum.; Authors - Han Sun
human	WB	Front Cell Dev Biol; IL-18-Mediated SLC7A5 Overexpression Enhances Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells via the c-MYC Pathway.; Authors - Feifei Ni

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Charlotte (Verified Customer) (07-25-2024)

I tested the antibody with the following protocol. The most intense band corresponds to ALP. There is a lot of background. Maybe cell line specific as C3H10T1/2 cells are differentiating into the osteoblast lineage upon stimulation ? Blocking 1h in 5% milk in TBST primary incubation 1/1000 in blocking solution, O.N. at 4°C wash 5*5min TBST secondary incubation 1/5000 in blocking solution, 1h at RT° wash 4*5min PBST + 5min PBS

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: C3H10T1/2

ALPL Antibody Western Blot validation (1/1000 dilution) in C3H10T1/2 (Cat no:11187-1-AP)

FH

CHAO (Verified Customer) (03-31-2022)

Weak bands may due to tissue specificity

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

FH

Jie (Verified Customer) (01-23-2020)

Smear band but with the right product size on western blot. Immunohistochemical staining showed desired results in control versus knockout mouse model

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse colon tissue

FH

Jingwen (Verified Customer) (01-17-2020)

This antibody worked very well and showed the specific band around 70kDa in western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart section

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C