Anticorps Polyclonal de lapin anti-ALDH6A1

ALDH6A1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 20452-1-AP

Synonymes

ALDH6A1, MMSADHA, MMSDH

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Galerie de données de validation

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  WB analysis using 20452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 20452-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20452-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 10x lens).

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  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 40x lens).

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  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20452-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules HEK-293, tissu cardiaque de souris, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de cirrhose hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:12000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

20452-1-AP cible ALDH6A1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH6A1 Protéine recombinante Ag13879
• Nom complet: aldehyde dehydrogenase 6 family, member A1
• Masse moléculaire calculée: 535 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC004909
• Symbole du gène: ALDH6A1
• Identification du gène (NCBI): 4329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cancers (Basel); RNA Sequencing of Collecting Duct Renal Cell Carcinoma Suggests an Interaction between miRNA and Target Genes and a Predominance of Deregulated Solute Carrier Genes.; Authors - Sven Wach
human	WB	Front Neurol; Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.; Authors - Matthias Höllerhage
human	WB	Theranostics; DNMT1 mediates the disturbed flow-induced endothelial to mesenchymal transition through disrupting β-alanine and carnosine homeostasis; Authors - Jianan Zhao