Anticorps Polyclonal de lapin anti-ALDH4A1

• Phare
• Validé par KD/KO

ALDH4A1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11604-1-AP

Synonymes

ALDH4, ALDH4A1, P5C dehydrogenase, P5CD, P5CDh, P5CDhL, P5CDhS

---

Galerie de données de validation

• 

  WB analysis of human heart using 11604-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11604-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11604-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11604-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11604-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11604-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11604-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11604-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 11604-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11604-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 11604-1-AP

  IP result of anti-ALDH4A1 (IP:11604-1-AP, 4ug; Detection:11604-1-AP 1:800) with mouse skeletal muscle tissue lysate 3600ug.

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:100 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules HepG2, cellules K-562, tissu cardiaque de rat, tissu cérébral humain, tissu de muscle squelettique de souris, tissu de muscle squelettique humain, tissu d'intestin grêle de souris, tissu hépatique humain
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la prostate humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

11604-1-AP cible ALDH4A1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH4A1 Protéine recombinante Ag2147
• Nom complet: aldehyde dehydrogenase 4 family, member A1
• Masse moléculaire calculée: 563 aa, 62 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC023600
• Symbole du gène: ALDH4A1
• Identification du gène (NCBI): 8659
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH4A1(Aldehyde dehydrogenase family 4 member A1) is also named as ALDH4, P5CDH,P5C dehydrogenase and belongs to the aldehyde dehydrogenase family. P5CDH is a mitochondrial matrix NAD(+)-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate.Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2).

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.; Authors - Liu Wei W ; KD Validated
human	WB	Sci Rep; Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.; Authors - Bobby Jones
human,mouse	WB,IHC	J Exp Clin Cancer Res; Pharmacological targeting PTK6 inhibits the JAK2/STAT3 sustained stemness and reverses chemoresistance of colorectal cancer.; Authors - Chaoqun Liu
mouse	WB	Nat Metab; Inhibition of the proline metabolism rate-limiting enzyme P5CS allows proliferation of glutamine-restricted cancer cells; Authors - Samantha J Linder ; KD Validated