Anticorps Monoclonal anti-ALDH3A1

• Phare
• Validé par KD/KO

ALDH3A1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1D4G7

N° de cat : 68036-1-Ig

Synonymes

Aldehyde dehydrogenase 3, ALDH3, ALDH3A1, ALDHIII

---

Galerie de données de validation

• 

  WB analysis of A549 using 68036-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 68036-1-Ig

  WB result of ALDH3A1 antibody (68036-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH3A1 transfected A549 cells.

• 

  WB analysis of A549 using 68036-1-Ig

  WB result of ALDH3A1 antibody (68036-1-Ig; 1:80000; room temperature for 1.5 hours) with wild-type and ALDH3A1 knockout A549 cells.

• 

  WB analysis of LNCaP using 68036-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68036-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human saliva using 68036-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 68036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 68036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68036-1-Ig (ALDH3A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 68036-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ALDH3A1 antibody (68036-1-Ig, Clone: 1D4G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules LNCaP
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68036-1-Ig cible ALDH3A1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALDH3A1 Protéine recombinante Ag8125
• Nom complet: aldehyde dehydrogenase 3 family, memberA1
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC004370
• Symbole du gène: ALDH3A1
• Identification du gène (NCBI): 218
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).