• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-ALDH3A1

ALDH3A1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

N° de cat : 15578-1-AP

Synonymes

EC:1.2.1.5, ALDHIII, ALDH3, Aldehyde dehydrogenase, dimeric NADP-preferring, AL3A1



Applications testées

Résultats positifs en WBcellules A549,
Résultats positifs en IPcellules A549,
Résultats positifs en IF/ICCcellules HEK-293T,
Résultats positifs en FC (Intra)cellules HEK-293,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15578-1-AP cible ALDH3A1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, Lapin, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène ALDH3A1 Protéine recombinante Ag7977
Nom complet aldehyde dehydrogenase 3 family, memberA1
Masse moléculaire calculée 50 kDa
Poids moléculaire observé 48 kDa
Numéro d’acquisition GenBankBC004370
Symbole du gène ALDH3A1
Identification du gène (NCBI) 218
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).

Protocole

Product Specific Protocols
WB protocol for ALDH3A1 antibody 15578-1-APDownload protocol
IF protocol for ALDH3A1 antibody 15578-1-APDownload protocol
IP protocol for ALDH3A1 antibody 15578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.

Authors - Alessandra Fiore
ratIF

Nat Commun

Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma.

Authors - Bin Kong
humanIHC

Adv Healthc Mater

Sustained Release of TPCA-1 from Silk Fibroin Hydrogels Preserves Keratocyte Phenotype and Promotes Corneal Regeneration by Inhibiting Interleukin-1β Signaling.

Authors - Wei Zhang
humanWB

Cell Chem Biol

Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.

Authors - Amy Flor
humanIF

Antioxid Redox Signal

Elucidation of the AGR2 interactome in esophageal adenocarcinoma cells identifies a redox sensitive chaperone hub for the quality control of MUC-5AC.

Authors - Jack C Worfolk
humanIF,WB

ACS Biomater Sci Eng

Regulation of the Keratocyte Phenotype and Cell Behavior Derived from Human Induced Pluripotent Stem Cells by Substrate Stiffness

Authors - Lan Zhang