Anticorps Polyclonal de lapin anti-ALDH1L2

• Phare
• Validé par KD/KO

ALDH1L2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 21391-1-AP

Synonymes

ALDH1L2

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Galerie de données de validation

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  WB analysis using 21391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21391-1-AP (ALDH1L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21391-1-AP (ALDH1L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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  IF Staining of A431 using 21391-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ALDH1L2 antibody (21391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, tissu pancréatique de rat
• Résultats positifs en IHC: tissu pancréatique humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below

Informations sur le produit

21391-1-AP cible ALDH1L2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH1L2 Protéine recombinante Ag16046
• Nom complet: aldehyde dehydrogenase 1 family, member L2
• Masse moléculaire calculée: 923 aa, 102 kDa
• Poids moléculaire observé: 102 kDa, 89 kDa
• Numéro d’acquisition GenBank: BC103935
• Symbole du gène: ALDH1L2
• Identification du gène (NCBI): 160428
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH1L2(Aldehyde dehydrogenase family 1 member L2) is also named as mitochondrial 10-FTHFDH, mtFDH and belongs to the aldehyde dehydrogenase family and ALDH1L subfamily. The ALDH1L2 gene has three transcriptional variants with the molecular weight of 102 kDa, 42 kDa and 89 kDa(PMID:19823103). It is a mitochondrial enzyme, a likely source of CO2 production from 10-formyltetrahydrofolate in mitochondria and playing an essential role in the distribution of one-carbon groups between the cytosolic and mitochondrial compartments of the cell(PMID: 20498374). It also has dehydrogenase/hydrolase activities similar to cytosolic FDH (ALDH1L1). The native ALDH1L2 has been reported to exist as a dimer or tetramer(PMID:7822273).

Publications

Species	Application	Title
mouse	WB	Mol Cell; Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.; Authors - Alessandra Fiore
human	IHC	Sci Adv; G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.; Authors - Yang Zhang
human	WB	Nat Commun; Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.; Authors - Eduardo Balsa
pig	WB	Mol Metab; Maternal hyperglycemia induces alterations in hepatic amino acid, glucose and lipid metabolism of neonatal offspring: Multi-omics insights from a diabetic pig model; Authors - Bachuki Shashikadze
human,mouse	WB,IHC	Free Radic Biol Med; Ursodeoxycholic acid protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction through acting on ALDH1L2.; Authors - Yunwen Yang
human	WB	J Biol Chem; Acetylation of aldehyde dehydrogenase ALDH1L2 regulates cellular redox balance and the chemosensitivity of colorectal cancer to 5-Fluorouracil; Authors - Chaoqun Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (08-26-2019)

Works well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: liver, kidney, hippocampus

ALDH1L2 Antibody Western Blot, validation (1:1000 dilution) in liver, kidney, hippocampus (Cat no:21391-1-AP)