Anticorps Polyclonal de lapin anti-ALDH1A2

ALDH1A2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 13951-1-AP

Synonymes

ALDH1A2, RALDH 2, RALDH(II), RALDH2, RALDH2 T, Retinal dehydrogenase 2

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Galerie de données de validation

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  WB analysis of K-562 using 13951-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13951-1-AP (ALDH1A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13951-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13951-1-AP (ALDH1A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13951-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13951-1-AP (ALDH1A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13951-1-AP

  IP result of anti-ALDH1A2 (IP:13951-1-AP, 4ug; Detection:13951-1-AP 1:300) with mouse testis tissue lysate 4400ug.

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  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 5 publications below

Informations sur le produit

13951-1-AP cible ALDH1A2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH1A2 Protéine recombinante Ag5062
• Nom complet: aldehyde dehydrogenase 1 family, member A2
• Masse moléculaire calculée: 518 aa, 57 kDa
• Poids moléculaire observé: 53-57 kDa
• Numéro d’acquisition GenBank: BC030589
• Symbole du gène: ALDH1A2
• Identification du gène (NCBI): 8854
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	Elife; FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.; Authors - Carlos J Diaz Osterman
human	WB	iScience; Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression; Authors - Zhilei Zhang
mouse	WB	J Nutr Biochem; All-trans retinoic acid induces lipophagy through the activation of the AMPK-Beclin1 signaling pathway and reduces Rubicon expression in adipocytes; Authors - Yuki Mori
human	WB	Am J Cancer Res; Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression; Authors - Bing-Yen Wang
human	IHC	J Lipid Res; Importance of ALDH1A enzymes in determining human testicular retinoic acid concentrations.; Authors - Samuel L Arnold