Anticorps Polyclonal de lapin anti-P5CS

• Phare
• Validé par KD/KO

P5CS Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 17719-1-AP

Synonymes

ALDH18A1, GK, GPR, GSAS, P5CS, PYCS

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Galerie de données de validation

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  WB analysis using 17719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17719-1-AP (P5CS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17719-1-AP (P5CS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17719-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17719-1-AP (P5CS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17719-1-AP

  IP result of anti-P5CS (IP:17719-1-AP, 4ug; Detection:17719-1-AP 1:1000) with mouse brain tissue lysate 1600 ug.

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  IHC staining of human breast cancer using 17719-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17719-1-AP (P5CS antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17719-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17719-1-AP (P5CS antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 17719-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using P5CS antibody (17719-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

17719-1-AP cible P5CS dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P5CS Protéine recombinante Ag12100
• Nom complet: aldehyde dehydrogenase 18 family, member A1
• Masse moléculaire calculée: 795 aa, 87 kDa
• Poids moléculaire observé: 87 kDa
• Numéro d’acquisition GenBank: BC106054
• Symbole du gène: P5CS
• Identification du gène (NCBI): 5832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Sci Adv; Loss of attachment promotes proline accumulation and excretion in cancer cells; Authors - Steven E Pilley
human	WB	J Exp Med; Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.; Authors - Miao Liu ; KD Validated
mouse	WB	Autophagy; Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.; Authors - Jingru Bai
human	WB	Proc Natl Acad Sci U S A; Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.; Authors - Liu Wei W
mouse	WB	Int J Mol Sci; Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling; Authors - Jana Key
human	WB	Sci Rep; Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.; Authors - Wei Liu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristian (Verified Customer) (02-05-2020)

The cells were collected, then lyzed five minutes at 95C in SDS-loading buffer.The lysate was run on a 4-20% denaturing SDS-PAGE, then blotted unto a nitrocellulose membrane. The protein was detected using primary antibody diluted 1:1,000 in blotto (PBS, 5% milk, 0.1% Tween-20), then secondary in a 1:10,000 dilution in blotto.Detected using ECL reagents.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human kidney cancer cells (RCC4)

P5CS Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:17719-1-AP)