Anticorps Polyclonal de lapin anti-ALCAM

ALCAM Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 21972-1-AP

Synonymes

ALCAM, CD166, CD166 antigen, MEMD

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Galerie de données de validation

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  WB analysis using 21972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 21972-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21972-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21972-1-AP (ALCAM Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21972-1-AP (ALCAM Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules U2OS, tissu cérébral de souris, tissu hépatique humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral de souris, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

21972-1-AP cible ALCAM dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALCAM Protéine recombinante Ag16892
• Nom complet: activated leukocyte cell adhesion molecule
• Masse moléculaire calculée: 583 aa, 65 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC137097
• Symbole du gène: ALCAM
• Identification du gène (NCBI): 214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

Publications

Species	Application	Title
human	WB	Cancer Discov; Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer; Authors - Nurmaa K Dashzeveg
human	IHC	EBioMedicine; Organoid modelling identifies that DACH1 functions as a tumour promoter in colorectal cancer by modulating BMP signalling.; Authors - Xiang Hu
human	WB,IF	Onco Targets Ther; Alpha-1 Antitrypsin Induces Epithelial-to-Mesenchymal Transition, Endothelial-to-Mesenchymal Transition, and Drug Resistance in Lung Cancer Cells.; Authors - Dong-Ming Wu
human	WB,IHC	Cell Death Dis; PMID: 31024010; Authors - Dong-Ming Wu