Anticorps Monoclonal anti-Albumin

Albumin Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

4A1C11

N° de cat : 66051-1-Ig

Synonymes

Alb, albumin, HSA, Serum albumin


Filter:
  • Western Blot (WB) analysis of human plasma using Albumin Monoclonal antibody (66051-1-Ig)

    WB analysis of human plasma using 66051-1-Ig

    human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with 66051-1-Ig (Albumin Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    WB analysis of human liver using 66051-1-Ig

    human liver tissue were subjected to SDS PAGE followed by western blot with 66051-1-Ig (ALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IP experiment of HepG2 using 66051-1-Ig

    IP result of anti-Albumin(IP:66051-1-Ig, 4ug; Detection:66051-1-Ig 1:1000) with HepG2 cells lysate 1720 ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IHC staining of human liver using 66051-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IHC staining of human liver using 66051-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IF Staining of HepG2 using 66051-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IF Staining of HepG2 using 66051-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

"Albumin Antibodies" Comparison

View side-by-side comparison of Albumin antibodies from other vendors to find the one that best suits your research needs.

Applications testées

Résultats positifs en WBtissu plasmatique humain, tissu hépatique humain
Résultats positifs en IHCtissu hépatique humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66051-1-Ig cible Albumin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

Réactivité Humain, porc, rat
Réactivité citéerat, Humain, Lapin, porc, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Albumin Protéine recombinante Ag9885
Nom complet albumin
Masse moléculaire calculée 609 aa, 69 kDa
Poids moléculaire observé 66 kDa
Numéro d’acquisition GenBankBC034023
Symbole du gène ALB
Identification du gène (NCBI) 213
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.

Protocole

Product Specific Protocols
WB protocol for Albumin antibody 66051-1-IgDownload protocol
IHC protocol for Albumin antibody 66051-1-IgDownload protocol
IF protocol for Albumin antibody 66051-1-IgDownload protocol
IP protocol for Albumin antibody 66051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
humanIF

J Hepatol

Targeting NFATc4 attenuates non-alcoholic steatohepatitis in mice.

Authors - Meng Du
pigIF

Sci Bull (Beijing)

Single-cell dynamics of liver development in postnatal pigs

Authors - Lin Rao
humanWB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
humanWB

Small

Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes.

Authors - Ji-Qian Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yuan (Verified Customer) (10-15-2019)

This Albumin antibody is working very well on human seminal plasma and blood plasma.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Human seminal plasma and blood plasma