Anticorps Polyclonal de lapin anti-AKAP3

AKAP3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 13907-1-AP

Synonymes

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein

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Galerie de données de validation

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  WB analysis using 13907-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13907-1-AP (AKAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 13907-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13907-1-AP (AKAP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13907-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13907-1-AP (AKAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13907-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13907-1-AP (AKAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 13907-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (13907-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse testis using 13907-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (13907-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat
• Résultats positifs en IHC: tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13907-1-AP cible AKAP3 dans les applications de WB, IHC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKAP3 Protéine recombinante Ag4885
• Nom complet: A kinase (PRKA) anchor protein 3
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC047535
• Symbole du gène: AKAP3
• Identification du gène (NCBI): 10566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness.; Authors - Tong Liu
mouse	WB	Proc Natl Acad Sci U S A; SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.; Authors - Haruhiko Miyata
mouse	IF	Development; The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development; Authors - Anja Beckers
mouse	WB,IF	J Genet Genomics; Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice; Authors - Yaling Wang
mouse	IF	Development; The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development.; Authors - Anja Beckers
pig	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu