Anticorps Polyclonal de lapin anti-AK3

AK3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12562-1-AP

Synonymes

adenylate kinase 3, AK 3, AK3, AK3L1, AK6, AKL3L, AKL3L1, FIX

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Galerie de données de validation

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  WB analysis of human placenta using 12562-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12562-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12562-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12562-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 12562-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12562-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12562-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12562-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12562-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12562-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12562-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12562-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12562-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12562-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12562-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12562-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12562-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12562-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12562-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12562-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12562-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12562-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12562-1-AP

  IP result of anti-AK3 (IP:12562-1-AP, 3ug; Detection:12562-1-AP 1:500) with mouse kidney tissue lysate 4400ug.

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  IHC staining of human pancreas cancer using 12562-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12562-1-AP (AK3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12562-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12562-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 12562-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12562-1-AP (AK3 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules BxPC-3, cellules HEK-293, cellules HepG2, cellules L02, cellules MCF-7, cellules Y79, tissu cardiaque de souris, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12562-1-AP cible AK3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AK3 Protéine recombinante Ag3146
• Nom complet: adenylate kinase 3
• Masse moléculaire calculée: 227 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC013771
• Symbole du gène: AK3
• Identification du gène (NCBI): 50808
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AK3(Adenylate kinase 3) is also named as AK3L1, AK6, AKL3L and belongs to the adenylate kinase family. It is located in the mitochondrial matrix and is a GTP:AMP phosphotransferase(PMID:2546792). AK3 can be detected AK3 at an apparent molecular mass of 28 kD by western blot analysis and subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction(PMID:11485571). This antibody may also recognize AK4 due to the high homology.

Publications

Species	Application	Title
human	WB	Mol Med Rep; MicroRNA‑214 upregulates HIF‑1α and VEGF by targeting ING4 in lung cancer cells.; Authors - Yue Li
human	WB	J Gene Med; MiR-96-5p promotes breast cancer migration by activating MEK/ERK signaling.; Authors - Wei-Yan Qin