Anticorps Polyclonal de lapin anti-Fetuin-A

Fetuin-A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16571-1-AP

Synonymes

A2HS, AHS, AHSG, alpha 2 HS glycoprotein, Alpha 2 Z globulin, Ba alpha 2 glycoprotein, FETUA, Fetuin A, Fetuin-A, HSGA

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Galerie de données de validation

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  WB analysis of human liver using 16571-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16571-1-AP (Fetuin-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16571-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16571-1-AP (Fetuin-A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16571-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16571-1-AP (Fetuin-A antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16571-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16571-1-AP (Fetuin-A antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, tissu cardiaque humain
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

16571-1-AP cible Fetuin-A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, Plasmodium falciparum
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Fetuin-A Protéine recombinante Ag9853
• Nom complet: alpha-2-HS-glycoprotein
• Masse moléculaire calculée: 367 aa, 39 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC048198
• Symbole du gène: Fetuin-A
• Identification du gène (NCBI): 197
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fetuin-A, also named as Alpha2-HS glycoprotein (AHSG), is a member of cystatin superfamily of protease inhibitors. It is a highly expressed glycoprotein in various fetal tissues whereas it is mainly expressed by the liver in adults. Fetuin A, an extracellular inhibitor of transforming growth factor β, is a profibrogenic stimulus in liver disease. Circulating fetuin-A may be a beneficial serum biomarker in the detection of liver and vascular fibrosis progression in patients with non-alcoholic fatty liver disease. It is also involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuin-A exerts its effects on the cardiovascular system by two different mechanisms. On the one hand it inhibits ins signaling and induces ins resistance contributing to the onset of atherosclerosis and on the other hand it inhibits calcium deposition and protects from vascular calcification.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification.; Authors - Xiao Lin
human	IHC, WB	Osteoarthritis Cartilage; Proteomics analysis of hip articular cartilage identifies differentially expressed proteins associated with osteonecrosis of the femoral head.; Authors - J Song
Plasmodium falciparum	WB	Malar J; The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.; Authors - Takahiro Tougan
human	IHC	Bioengineered; Alpha-2 Heremans Schmid Glycoprotein (AHSG) promotes the proliferation of bladder cancer cells by regulating the TGF-β signalling pathway.; Authors - Yufei Dong