Anticorps Polyclonal de lapin anti-AHSA1

• Phare
• Validé par KD/KO

AHSA1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14725-1-AP

Synonymes

AHA1, AHSA1, C14orf3

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Galerie de données de validation

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  WB analysis using 14725-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14725-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14725-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14725-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14725-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14725-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14725-1-AP (AHSA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14725-1-AP

  IP result of anti-AHSA1 (IP:14725-1-AP, 4ug; Detection:14725-1-AP 1:5000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human gliomas using 14725-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14725-1-AP (AHSA1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 14725-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14725-1-AP (AHSA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse skeletal muscle using 14725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14725-1-AP (AHSA1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 14725-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AHSA1 antibody (14725-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de gliome humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

14725-1-AP cible AHSA1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AHSA1 Protéine recombinante Ag6482
• Nom complet: AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
• Masse moléculaire calculée: 338 aa, 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC000321
• Symbole du gène: AHSA1
• Identification du gène (NCBI): 10598
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Front Immunol; The Prognostic and Immunotherapeutic Significance of AHSA1 in Pan-Cancer, and Its Relationship With the Proliferation and Metastasis of Hepatocellular Carcinoma.; Authors - Wenli Li ; KO Validated
human	WB	Cancers (Basel); AHSA1 Promotes Proliferation and EMT by Regulating ERK/CALD1 Axis in Hepatocellular Carcinoma; Authors - Jiakang Zhang ; KD Validated
human,mouse	WB,IHC	J Hepatocell Carcinoma; AHSA1 Regulates Hepatocellular Carcinoma Progression via the TGF-β/Akt-Cyclin D1/CDK6 Pathway; Authors - Yanjun Gao