Anticorps Polyclonal de lapin anti-AHNAK

AHNAK Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 16637-1-AP

Synonymes

AHNAK, AHNAK nucleoprotein, AHNAKRS, Desmoyokin, PM227

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Galerie de données de validation

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  WB analysis using 16637-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 16637-1-AP (AHNAK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 16637-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16637-1-AP (AHNAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 16637-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16637-1-AP (AHNAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 16637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16637-1-AP (AHNAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16637-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHNAK antibody (16637-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu cutané de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

16637-1-AP cible AHNAK dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AHNAK Protéine recombinante Ag9986
• Nom complet: AHNAK nucleoprotein
• Masse moléculaire calculée: 629 kDa
• Numéro d’acquisition GenBank: BC000926
• Symbole du gène: AHNAK
• Identification du gène (NCBI): 79026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AHNAK, also known as desmoyokin, is described as a giant scaffold protein based on its large size (629 kDa) and ability to interact with different proteins to form multi-protein complexes. The bulk of the protein is assembled in 128-residue repetitive elements known as the central repeated unit (CRU). Its proposed functions are quite diverse, ranging from a role in the formation of the blood brain barrier, in cell architecture and migration, to the regulation of cardiac channels and muscle membrane repair. AHNAK is differentially expressed in some cancer cell lines. The expression of AHNAK is subsequently localized to the plasma membrane of keratinocytes in human epidermis. AHNAK has been reported in many intracellular locations including the nucleus, cytoplasm and plasma membrane.

Publications

Species	Application	Title
human	IF	Nat Commun; Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.; Authors - Yuta Asano
human	IF	EMBO Rep; Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging; Authors - Monami Ogura
human	FC	Clin Exp Immunol; The AHNAK induces increased IL-6 production in CD4+T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss.; Authors - Liman Li