Anticorps Polyclonal de lapin anti-Angiotensinogen

Angiotensinogen Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 23972-1-AP

Synonymes

AGT, Serpin A8, SERPINA8, Ang IV, Ang III

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Galerie de données de validation

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  WB analysis using 23972-1-AP

  Human plasma were subjected to SDS PAGE followed by western blot with 23972-1-AP (Angiotensinogen antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23972-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23972-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 23972-1-AP

  Immunofluorescent analysis of HepG2 cells using 23972-1-AP (Angiotensinogen antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HEK-293 using 23972-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Angiotensinogen antibody (23972-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of L02 using 23972-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using Angiotensinogen antibody (23972-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HeLa using 23972-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Angiotensinogen (23972-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: plasma humain,
• Résultats positifs en IHC: tissu hépatique humain, tissu de cirrhose hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293, cellules HepG2, cellules L02
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

23972-1-AP cible Angiotensinogen dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Angiotensinogen Protéine recombinante Ag21121
• Nom complet: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Masse moléculaire calculée: 485 aa, 53 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC011519
• Symbole du gène: AGT
• Identification du gène (NCBI): 183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Publications

Species	Application	Title
mouse	IF	JCI Insight; Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits.; Authors - Wen Ding