Anticorps Polyclonal de lapin anti-GPAT3

• Phare
• Validé par KD/KO

GPAT3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 20603-1-AP

Synonymes

AGPAT9, 1-AGPAT 9, 1-AGP acyltransferase 9, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10

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Galerie de données de validation

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  WB analysis of rat kidney using 20603-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 20603-1-AP (AGPAT9 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20603-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20603-1-AP (AGPAT9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 20603-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20603-1-AP (AGPAT9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20603-1-AP (AGPAT9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20603-1-AP (AGPAT9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20603-1-AP (AGPAT9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20603-1-AP (AGPAT9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20603-1-AP (AGPAT9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20603-1-AP (AGPAT9 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20603-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AGPAT9 antibody (20603-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 20603-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human AGPAT9 (20603-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu rénal de rat, cellules HeLa, cellules HT-1080
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Informations sur le produit

20603-1-AP cible GPAT3 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPAT3 Protéine recombinante Ag14579
• Nom complet: 1-acylglycerol-3-phosphate O-acyltransferase 9
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC006236
• Symbole du gène: AGPAT9
• Identification du gène (NCBI): 84803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPAT3, also known as AGPAT9, is a member of the lysophosphatidic acid acyltransferase protein family. GPAT3 is an enzyme that catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
mouse	WB	Hum Mol Genet; PMID: 31873720; Authors - Mingming Gao ; KO Validated
mouse	WB,IF	Cell Death Dis; GPAT3 regulates the synthesis of lipid intermediate LPA and exacerbates Kupffer cell inflammation mediated by the ERK signaling pathway; Authors - Guoqiang Fan
human	WB	Ecotoxicol Environ Saf; Perturbation of arachidonic acid and glycerolipid metabolism promoted particulate matter-induced inflammatory responses in human bronchial epithelial cells; Authors - Jian Wang ; KD Validated
mouse	WB	Biomed Pharmacother; Si-Ni-San reverses dietary fat absorption defects in a murine model of depression; Authors - Yuanyuan Liu