Anticorps Polyclonal de lapin anti-AGPAT6

AGPAT6 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16762-1-AP

Synonymes

1 AGP acyltransferase 6, 1 AGPAT 6, AGPAT6, GPAT4, LPAAT zeta, LPAATZ, TSARG7

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Galerie de données de validation

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  WB analysis using 16762-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16762-1-AP (AGPAT6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16762-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16762-1-AP (AGPAT6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16762-1-AP

  IP result of anti-AGPAT6 (IP:16762-1-AP, 4ug; Detection:16762-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

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  IHC staining of human testis using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16762-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

16762-1-AP cible AGPAT6 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AGPAT6 Protéine recombinante Ag10294
• Nom complet: 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acid acyltransferase, zeta)
• Masse moléculaire calculée: 456 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC051377
• Symbole du gène: AGPAT6
• Identification du gène (NCBI): 137964
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AGPAT6 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family. AGPAT6 is expressed in brown adipose tissue and mammary epithelium as well as many other tissues. A deficiency of AGPAT6 in mice results in resistance to dietinduced and genetic forms of obesity.

Publications

Species	Application	Title
human	WB	Invest Ophthalmol Vis Sci; Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves.; Authors - Sasha M Milbeck
mouse	WB	EMBO Rep; MTCH2 cooperates with MFN2 and lysophosphatidic acid synthesis to sustain mitochondrial fusion; Authors - Andres Goldman