Anticorps Polyclonal de lapin anti-AGL

• Phare
• Validé par KD/KO

AGL Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 16582-1-AP

Synonymes

AGL, Amylo 1,6 glucosidase, Dextrin 6 alpha D glucosidase, GDE, Glycogen debranching enzyme

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Galerie de données de validation

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  WB analysis using 16582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16582-1-AP (AGL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16582-1-AP

  WB result of AGL antibody (16582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGL transfected HEK-293 cells.

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  WB analysis of mouse heart using 16582-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16582-1-AP (AGL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 16582-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16582-1-AP (AGL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16582-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16582-1-AP (AGL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

16582-1-AP cible AGL dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AGL Protéine recombinante Ag9962
• Nom complet: amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
• Masse moléculaire calculée: 1532 aa, 175 kDa
• Poids moléculaire observé: 165 kDa
• Numéro d’acquisition GenBank: BC078663
• Symbole du gène: AGL
• Identification du gène (NCBI): 178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III; Authors - Antoine Gardin
rat	WB	Carbohydr Polym; Liver fibrosis alters the molecular structures of hepatic glycogen; Authors - Yujun Wan
human	WB	iScience; MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.; Authors - Dong-Xue Ding
rat	WB	PLoS One; A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise; Authors - Seong Eun Kwak
mouse	WB	JCI Insight; Synergism of dual AAV gene therapy and rapamycin rescues GSDIII phenotype in muscle and liver; Authors - Louisa Jauze
human	WB	Stem Cell Res; Generation of three induced pluripotent stem cell lines from patients with glycogen storage disease type III; Authors - Lucille Rossiaud

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jeremy (Verified Customer) (09-08-2019)

Highly sensitive for the detection of Agl protein in different tissue including Heart, Diaphragm and different muscles

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Diaphragm

AGL Antibody Western Blot, validation (1/1000 dilution) in Diaphragm (Cat no:16582-1-AP)