Anticorps Polyclonal de lapin anti-AFG3L2

• Phare
• Validé par KD/KO

AFG3L2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

27

Avis (1)

N° de cat : 14631-1-AP

Synonymes

AFG3 like protein 2, AFG3L2, Paraplegin like protein

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Galerie de données de validation

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  WB analysis using 14631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14631-1-AP

  WB result of AFG3L2 antibody (14631-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFG3L2 transfected HeLa cells.

• 

  WB analysis using 14631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14631-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14631-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14631-1-AP

  IP result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:5000) with HeLa cells lysate 1560 ug.

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  IHC staining of human kidney using 14631-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 14631-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 14631-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14631-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14631-1-AP

  Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 14631-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 25 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

14631-1-AP cible AFG3L2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AFG3L2 Protéine recombinante Ag6209
• Nom complet: AFG3 ATPase family gene 3-like 2 (yeast)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC065016
• Symbole du gène: AFG3L2
• Identification du gène (NCBI): 10939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).

Publications

Species	Application	Title
mouse	WB	Mol Cell; Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex; Authors - Chen-Wei Tsai ; KD Validated
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
human	IF	Cancer Res; Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition; Authors - Liang Fang
mouse	WB	Research (Wash D C); LonP1 Links Mitochondria-ER Interaction to Regulate Heart Function; Authors - Yujie Li
human	WB	Brain; Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.; Authors - Gerald Pfeffer

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-28-2023)

clean band around the expected size

• Applications: Western Blot