Anticorps Polyclonal de lapin anti-ADHFE1

ADHFE1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 19359-1-AP

Synonymes

ADH8, ADHFE1, HMFT2263, HOT

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Galerie de données de validation

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  WB analysis of mouse heart using 19359-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 19359-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19359-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 19359-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19359-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 19359-1-AP

  IP result of anti-ADHFE1 (IP:19359-1-AP, 4ug; Detection:19359-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of human colon using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19359-1-AP (ADHFE1 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human liver using 19359-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19359-1-AP (ADHFE1 Antibody) at dilution of 1:50(under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du côlon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19359-1-AP cible ADHFE1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADHFE1 Protéine recombinante Ag6803
• Nom complet: alcohol dehydrogenase, iron containing, 1
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC064634
• Symbole du gène: ADHFE1
• Identification du gène (NCBI): 137872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.