Anticorps Polyclonal de lapin anti-ADAR1

ADAR1 Polyclonal Antibody for WB, IP, IHC, ELISA, IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, IHC, CoIP, ELISA, IF/ICC

Conjugaison

Non conjugué

Publications

17

N° de cat : 14330-1-AP

Synonymes

ADAR, 136 kDa double-stranded RNA-binding protein, DSH, DRADA, Double-stranded RNA-specific adenosine deaminase

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Galerie de données de validation

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  WB analysis of HeLa using 14330-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14330-1-AP (ADAR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14330-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14330-1-AP (ADAR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14330-1-AP

  IP result of anti-ADAR1 (IP:14330-1-AP, 4ug; Detection:14330-1-AP 1:800) with HepG2 cells lysate 1085 ug.

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  IHC staining of human gliomas using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14330-1-AP (ADAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14330-1-AP

  Immunofluorescent analysis of HepG2 cells, using ADAR antibody 14330-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

14330-1-AP cible ADAR1 dans les applications de WB, IP, IHC, CoIP, ELISA, IF/ICC et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAR1 Protéine recombinante Ag5609
• Nom complet: adenosine deaminase, RNA-specific
• Masse moléculaire calculée: 136 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC038227
• Symbole du gène: ADAR1
• Identification du gène (NCBI): 103
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).

Publications

Species	Application	Title
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
human	WB	Neurobiol Stress; Roles of miR-432 and circ_0000418 in mediating the anti-depressant action of ADAR1.; Authors - Xiaonan Zhang
human	IHC	Am J Cancer Res; The aberrant expression of ADAR1 promotes resistance to BET inhibitors in pancreatic cancer by stabilizing c-Myc.; Authors - Yan Sun
human	WB	Toxicol Appl Pharmacol; The anti-cancer drug candidate CBL0137 induced necroptosis via forming left-handed Z-DNA and its binding protein ZBP1 in liver cells; Authors - Jun Li
human	WB	Life Sci; The RNA editing enzyme ADAR modulated by the rs1127317 genetic variant diminishes EGFR-TKIs efficiency in advanced lung adenocarcinoma.; Authors - Hui Hua
mouse	IHC	Toxicol Lett; Decrease in ADAR1 expression by exposure to cigarette smoke enhances susceptibility to oxidative stress.; Authors - Masashi Takizawa