Anticorps Polyclonal de lapin anti-ADAMTS12

• Phare
• Validé par KD/KO

ADAMTS12 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 24934-1-AP

Synonymes

ADAM TS 12, ADAM TS12, ADAMTS 12, ADAMTS12, PRO4389

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Galerie de données de validation

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  WB analysis of mouse placenta using 24934-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 24934-1-AP (ADAMTS12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24934-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24934-1-AP (ADAMTS12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 24934-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ADAMTS12 antibody (24934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu placentaire de souris, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IF: See 1 publications below

Informations sur le produit

24934-1-AP cible ADAMTS12 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAMTS12 Protéine recombinante Ag20712
• Nom complet: ADAM metallopeptidase with thrombospondin type 1 motif, 12
• Masse moléculaire calculée: 178 kDa
• Poids moléculaire observé: 155 kDa
• Numéro d’acquisition GenBank: BC058841
• Symbole du gène: ADAMTS12
• Identification du gène (NCBI): 81792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADAMTS12(A disintegrin and metalloproteinase with thrombospondin motifs 12) is also named as AT12, ADAM-TS12, PRO4389 and belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS7 and ADAMTS12 appear to be potent negative regulators of endplate cartilage development(PMID:22247065). This protein has 3 isoforms produced by alternative splicing. The full length protein has 14 glycosylation sites.

Publications

Species	Application	Title
human	WB	Oncotarget; Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells.; Authors - Tania Fontanil
human	WB,IF	Hum Mol Genet; Epigenetic changes in reterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth.; Authors - Sneha Mani ; KD Validated
human	WB	Cell Signal; ADAMTS12 promotes oxaliplatin chemoresistance and angiogenesis in gastric cancer through VEGF upregulation; Authors - Yingming Jiang
human	WB	Sci Rep; ADAMTS12 acts as a tumor microenvironment related cancer promoter in gastric cancer.; Authors - Yangming Hou ; KD Validated
human	WB	Int J Mol Med; Overexpression of PRDM13 inhibits glioma cells via Rho and GTP enzyme activation protein.; Authors - Linna Zhang
human	WB	Hepatobiliary Pancreat Dis Int; ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer.; Authors - Rui-Zhe He ; KD Validated