Anticorps Polyclonal de lapin anti-ADAM2

ADAM2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 13952-1-AP

Synonymes

ADAM 2, ADAM metallopeptidase domain 2, ADAM2, Cancer/testis antigen 15, CRYN1, CRYN2, CT15, Fertilin subunit beta, FTNB, PH 30, PH 30b, PH30, PH30 beta

---

Galerie de données de validation

• 

  WB analysis of mouse testis using 13952-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13952-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 13952-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13952-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13952-1-AP (ADAM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13952-1-AP cible ADAM2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAM2 Protéine recombinante Ag5063
• Nom complet: ADAM metallopeptidase domain 2
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 50-55 kDa, 65-75 kDa
• Numéro d’acquisition GenBank: BC034957
• Symbole du gène: ADAM2
• Identification du gène (NCBI): 2515
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction(PMID: 27341348). Fertilin, a heterodimeric protein complex composed of a(ADAM1) and b(ADAM2) subunits present on the sperm surface, has been believed to mediate adhesion and fusion between the sperm and egg plasma membranes. ADAM2-deficient mouse sperm is also defective in adhesion to and fusion with the egg membrane (PMID:16407235). It has 2 isoforms produced by alternative splicing and 5 glycosylation sites.