Anticorps Polyclonal de lapin anti-ADA

ADA Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (2)

N° de cat : 13328-1-AP

Synonymes

ADA, Adenosine aminohydrolase, adenosine deaminase

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Galerie de données de validation

• 

  WB analysis of HeLa using 13328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13328-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 13328-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse small intestine using 13328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 13328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules Sp2/0
• Résultats positifs en IHC: tissu d'estomac humain, tissu d'amygdalite humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'intestin grêle de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

13328-1-AP cible ADA dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADA Protéine recombinante Ag4128
• Nom complet: adenosine deaminase
• Masse moléculaire calculée: 363 aa, 41 kDa
• Poids moléculaire observé: 45-47 kDa
• Numéro d’acquisition GenBank: BC040226
• Symbole du gène: ADA
• Identification du gène (NCBI): 100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
mouse	WB	Eur J Pharmacol; Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.; Authors - Gerald Wölkart
mouse	WB,IHC	Cell Death Dis; Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation; Authors - Mei-Xin Li
human	WB	bioRxiv; The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma; Authors - Ying-Jiun C Chen
	WB	NPJ Biofilms Microbiomes; Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model; Authors - Yang Fu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Neta (Verified Customer) (07-28-2022)

antibody worked, signal slightly different than reported but overall OK.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse small intestine

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with NaCit antigen retrieval ph=6.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (neurons) at 3dpf

ADA Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (neurons) at 3dpf (Cat no:13328-1-AP)