Anticorps Polyclonal de lapin anti-ACPP

ACPP Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15840-1-AP

Synonymes

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

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Galerie de données de validation

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  WB analysis of PC-3 using 15840-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15840-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 15840-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15840-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15840-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15840-1-AP (ACPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15840-1-AP (ACPP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15840-1-AP (ACPP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 15840-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15840-1-AP (ACPP antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-3 using 15840-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 15840-1-AP (ACPP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules COLO 320, cellules MCF-7, tissu pulmonaire de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

15840-1-AP cible ACPP dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACPP Protéine recombinante Ag8592
• Nom complet: acid phosphatase, prostate
• Masse moléculaire calculée: 418 aa, 48 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC007460
• Symbole du gène: ACPP
• Identification du gène (NCBI): 55
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase, thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

Publications

Species	Application	Title
chicken	IHC	Biol Reprod; Avian Prostatic Acid Phosphatase: Estrogen Regulation in the Oviduct and Epithelial Cell-Derived Ovarian Carcinomas.; Authors - Hyocheol Bae
mouse	IHC	Am J Pathol; PIK3CG Is a Potential Therapeutic Target in Androgen Receptor-Indifferent Metastatic Prostate Cancer.; Authors - Wen-Cheng Chung
mouse	WB,IF,IP	Eur J Pain; Electroacupuncture relieves neuropathic pain by inhibiting degradation of the ecto-nucleotidase PAP in the dorsal root ganglions of CCI mice.; Authors - Xiaoxin Zhou
Rat	IHC, IF	Biochem Biophys Res Commun; Dietary cholesterol affects expression of prostatic acid phosphatase in reproductive organs of male rats.; Authors - Whasun Lim