Anticorps Polyclonal de lapin anti-ACOT9

• Phare
• Validé par KD/KO

ACOT9 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15901-1-AP

Synonymes

ACATE2, ACOT9, Acyl CoA thioester hydrolase 9, acyl CoA thioesterase 9, CGI 16, MT ACT48

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Galerie de données de validation

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  WB analysis using 15901-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACOT9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15901-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15901-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15901-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15901-1-AP (ACTO9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15901-1-AP (ACOT9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15901-1-AP (ACOT9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15901-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15901-1-AP (ACTO9 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7, tissu cardiaque de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu rénal de rat, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

15901-1-AP cible ACOT9 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACOT9 Protéine recombinante Ag8730
• Nom complet: acyl-CoA thioesterase 9
• Masse moléculaire calculée: 24 kDa, 49 kDa
• Poids moléculaire observé: 43-50 kDa
• Numéro d’acquisition GenBank: BC012573
• Symbole du gène: ACOT9
• Identification du gène (NCBI): 23597
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.

Publications

Species	Application	Title
human	IHC	Liver Int; Acyl-CoA thioesterase 9 promotes tumor growth and metastasis through reprogramming of fatty acid metabolism in hepatocellular carcinoma; Authors - Bao Wang ; KD Validated
mouse	WB	EMBO Mol Med; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy; Authors - Nneka Southwell