Anticorps Polyclonal de lapin anti-ACOT7

• Phare
• Validé par KD/KO

ACOT7 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15972-1-AP

Synonymes

ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1

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Galerie de données de validation

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  WB analysis using 15972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15972-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15972-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15972-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15972-1-AP (ACOT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

15972-1-AP cible ACOT7 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACOT7 Protéine recombinante Ag8759
• Nom complet: acyl-CoA thioesterase 7
• Masse moléculaire calculée: 370 aa, 40 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC017365
• Symbole du gène: ACOT7
• Identification du gène (NCBI): 11332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:10578051).  Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:10578051). May play an important physiological function in brain (PubMed:10578051). ACOT7 has 7 isoforms between 27-40 kDa, we can detect a band at 37 kDa. ACOT7 is high homology with ACOT7L, this antibody may also detect ACOT7L.

Publications

Species	Application	Title
		Nat Commun; Convergent genomic signatures of flight loss in birds suggest a switch of main fuel.; Authors - Shengkai Pan
human	WB,IHC	J Hepatocell Carcinoma; Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma.; Authors - Xingming Xie
mouse	WB	J Proteomics; TMT-labeled quantitative malonylome analysis on the longissimus dorsi muscle of Laiwu pigs reveals the role of ACOT7 in fat deposition; Authors - Wenlei Wang
mouse	WB	In Vitro Cell Dev Biol Anim; ACOT7 protects epidermal stem cells against lipid peroxidation; Authors - Guang Zhang
human	WB	BMC Mol Cell Biol; ARNTL2 upregulation of ACOT7 promotes NSCLC cell proliferation through inhibition of apoptosis and ferroptosis; Authors - Tao Wang ; KD Validated