Anticorps Polyclonal de lapin anti-ACE

ACE Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

25

N° de cat : 24743-1-AP

Synonymes

ACE, ACE1, Angiotensin converting enzyme, CD143, DCP, DCP1, Dipeptidyl carboxypeptidase I, Kininase II, MVCD3

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Galerie de données de validation

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  WB analysis of mouse heart using 24743-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 24743-1-AP (ACE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 24743-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 24743-1-AP (ACE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 24743-1-AP

  IP result of anti-ACE (IP:24743-1-AP, 4ug; Detection:24743-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human lung using 24743-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 24743-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 24743-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 24743-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 24743-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 24743-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24743-1-AP (ACE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris,
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 19 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

24743-1-AP cible ACE dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACE Protéine recombinante Ag20654
• Nom complet: angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
• Masse moléculaire calculée: 150 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: BC036375
• Symbole du gène: ACE
• Identification du gène (NCBI): 1636
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACE(Angiotensin-converting enzyme) is also named as DCP, DCP1 and belongs to the peptidase M2 family. It is involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. ACE also has a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. It has 4 isoforms produced by alternative splicing. The full length protein has 15 glycosylation sites and it can show a molecular weight of 170 kDa(PMID:16804085).

Publications

Species	Application	Title
human,rat	WB,IHC	Oxid Med Cell Longev; Tissue Renin-Angiotensin System (tRAS) Induce Intervertebral Disc Degeneration by Activating Oxidative Stress and Inflammatory Reaction.; Authors - Kaiqiang Sun
rat	IHC	J Cell Mol Med; PMID: 32073745; Authors - Jianxia Wen
mouse	WB	Diabetol Metab Syndr; Celastrol attenuates streptozotocin-induced diabetic cardiomyopathy in mice by inhibiting the ACE / Ang II / AGTR1 signaling pathway; Authors - Xuyong Zhao
mouse	IHC,WB	ACS Omega; Azelaic Acid Regulates the Renin-Angiotensin System and Improves Colitis Based on Network Pharmacology and Experimentation; Authors - Yangjie Liao
mouse,rat	WB	Cardiovasc Drugs Ther; Non-Invasive Local Acoustic Therapy Ameliorates Diabetic Heart Fibrosis by Suppressing ACE-Mediated Oxidative Stress and Inflammation in Cardiac Fibroblasts.; Authors - Liqing Weng
human	IF	Front Endocrinol (Lausanne); TMPRSS2 Expression and Activity Modulation by Sex-Related Hormones in Lung Calu-3 Cells: Impact on Gender-Specific SARS-CoV-2 Infection.; Authors - Donatella Treppiedi