- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-ACADL-Specific
ACADL-Specific Polyclonal Antibody for WB, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (3)
Applications
WB, IP, IF, IHC, ELISA
Conjugaison
Non conjugué
N° de cat : 17526-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | tissu rénal de souris, tissu cardiaque de souris, tissu hépatique de souris, tissu pulmonaire de souris |
Résultats positifs en IP | tissu rénal de souris, |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 3 publications below |
WB | See 44 publications below |
IHC | See 5 publications below |
IF | See 2 publications below |
IP | See 4 publications below |
Informations sur le produit
17526-1-AP cible ACADL-Specific dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | rat, Humain, porc, souris, Brebis |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | acyl-Coenzyme A dehydrogenase, long chain |
Masse moléculaire calculée | 48 kDa |
Poids moléculaire observé | 48 kDa |
Numéro d’acquisition GenBank | NM_001608 |
Symbole du gène | ACADL |
Identification du gène (NCBI) | 33 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.
Protocole
Product Specific Protocols | |
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WB protocol for ACADL-Specific antibody 17526-1-AP | Download protocol |
IP protocol for ACADL-Specific antibody 17526-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Int J Biol Sci Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma | ||
Diabetes Pivotal Role of O-GlcNAc Modification in Cold-Induced Thermogenesis by Brown Adipose Tissue Through Mitochondrial Biogenesis. | ||
Phytomedicine Shen-Shuai-II-Recipe inhibits tubular inflammation by PPARα-mediated fatty acid oxidation to attenuate fibroblast activation in fibrotic kidneys | ||
Cell Rep HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.
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Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Rebeca (Verified Customer) (03-11-2024) | I had a great experience ordering this product and it arrived within a few days.
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FH Wu (Verified Customer) (04-10-2021) | LCAD antibody worked good in IF assay at 1:50 dilution.
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FH Tanusree (Verified Customer) (12-18-2019) | Product worked well in WB at 1:500 dilution
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