• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-ACADL-Specific

ACADL-Specific Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 17526-1-AP

Synonymes

ACAD4, ACADL, ACADL-Specific, LCAD



Applications testées

Résultats positifs en WBtissu rénal de souris, tissu cardiaque de souris, tissu hépatique de souris, tissu pulmonaire de souris
Résultats positifs en IPtissu rénal de souris,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

17526-1-AP cible ACADL-Specific dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, Humain, porc, souris, Brebis
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet acyl-Coenzyme A dehydrogenase, long chain
Masse moléculaire calculée 48 kDa
Poids moléculaire observé 48 kDa
Numéro d’acquisition GenBankNM_001608
Symbole du gène ACADL
Identification du gène (NCBI) 33
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.

Protocole

Product Specific Protocols
WB protocol for ACADL-Specific antibody 17526-1-APDownload protocol
IP protocol for ACADL-Specific antibody 17526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
mouseWB

Circ Res

NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.

Authors - Dan Tong
humanWB,IF

Int J Biol Sci

Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma

Authors - Jingwei Cai
mouseWB

Diabetes

Pivotal Role of O-GlcNAc Modification in Cold-Induced Thermogenesis by Brown Adipose Tissue Through Mitochondrial Biogenesis.

Authors - Natsuko Ohashi
ratWB

Phytomedicine

Shen-Shuai-II-Recipe inhibits tubular inflammation by PPARα-mediated fatty acid oxidation to attenuate fibroblast activation in fibrotic kidneys

Authors - Meng Wang
mouse, humanWB, IHC

Cell Rep

HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.

Authors - De Huang
  • KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rebeca (Verified Customer) (03-11-2024)

I had a great experience ordering this product and it arrived within a few days.

  • Applications: Western Blot
FH

Wu (Verified Customer) (04-10-2021)

LCAD antibody worked good in IF assay at 1:50 dilution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Liver
ACADL-Specific Antibody Immunofluorescence validation (1:50 dilution) in Liver (Cat no:17526-1-AP)
FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain