Anticorps Polyclonal de lapin anti-ABCG1

• Phare
• Validé par KD/KO

ABCG1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA, IF

Conjugaison

Non conjugué

Publications

44

Avis (1)

N° de cat : 13578-1-AP

Synonymes

ABC8, ABCG1, White protein homolog, WHITE1, WHT1

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 13578-1-AP

  WB result of ABCG1 antibody (13578-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCG1 transfected RAW 264.7 cells.

• 

  WB analysis using 13578-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13578-1-AP (ABCG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13578-1-AP

  IP result of anti-ABCG1 (IP:13578-1-AP, 4ug; Detection:13578-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse placenta using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 13578-1-AP (ABCG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13578-1-AP (ABCG1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13578-1-AP (ABCG1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse placenta using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 13578-1-AP (ABCG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13578-1-AP (ABCG1 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, cellules HepG2, cellules RAW 264.7, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu placentaire de souris, tissu d'amygdalite humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 40 publications below
• IHC: See 6 publications below
• IF: See 6 publications below

Informations sur le produit

13578-1-AP cible ABCG1 dans les applications de WB, IHC, IP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABCG1 Protéine recombinante Ag4479
• Nom complet: ATP-binding cassette, sub-family G (WHITE), member 1
• Masse moléculaire calculée: 678 aa, 76 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC029158
• Symbole du gène: ABCG1
• Identification du gène (NCBI): 9619
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)

Publications

Species	Application	Title
human	WB	Cancer Res; PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer; Authors - Takehiro Yamamoto
mouse	WB	Autophagy; Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells.; Authors - Jun Matsuda
human	WB	Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
mouse	WB,IHC	J Control Release; Artemisinin and Procyanidins loaded multifunctional nanocomplexes alleviate atherosclerosis via simultaneously modulating lipid influx and cholesterol efflux.; Authors - Hongyan Zhou
mouse	WB	Br J Pharmacol; Targeting macrophage liver X receptors by hydrogel-encapsulated T0901317 reduces atherosclerosis without effect on hepatic lipogenesis.; Authors - Chuanrui Ma
mouse	IF	Carbohydr Polym; Cyclodextrin boostered-high density lipoprotein for antiatherosclerosis by regulating cholesterol efflux and efferocytosis.; Authors - Yanyan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shiford (Verified Customer) (09-24-2023)

We sorted cancer stem cell subpopulations from multiple cell lines by flow cytometry and used a cluster of antibodies related to stem cell characteristics to detect. ABCG1 is one of the possible stem cell markers, with clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1：1500
• Cell Tissue Type: Cancer cell lines from various tissues

ABCG1 Antibody Western Blot validation (1：1500 dilution) in Cancer cell lines from various tissues (Cat no:13578-1-AP)

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