Anticorps Polyclonal de lapin anti-ABCD1

• Phare
• Validé par KD/KO

ABCD1 Polyclonal Antibody for WB, IP, IF, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 18138-1-AP

Synonymes

ABCD1, ALD, ATP binding cassette sub family D member 1, ATP-binding cassette sub-family D member 1, EC:3.1.2.-, ABCD 1

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Galerie de données de validation

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  WB analysis using 18138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18138-1-AP

  WB result of ABCD1 antibody (18138-1-AP, 1:600) with si-Control and si-ABCD1 transfected HeLa cells..

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  WB analysis of HepG2 using 18138-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18138-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18138-1-AP

  IP result of anti-ABCD1 (IP:18138-1-AP, 4ug; Detection:18138-1-AP 1:700) with HeLa cells lysate 3000 ug.

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  IF Staining of HeLa using 18138-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ABCD1 antibody (18138-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HeLa using 18138-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ABCD1 (18138-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

18138-1-AP cible ABCD1 dans les applications de WB, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABCD1 Protéine recombinante Ag12757
• Nom complet: ATP-binding cassette, sub-family D (ALD), member 1
• Masse moléculaire calculée: 745 aa, 83 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC025358
• Symbole du gène: ABCD1
• Identification du gène (NCBI): 215
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

Publications

Species	Application	Title
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
human	WB	Signal Transduct Target Ther; Structural insights into substrate recognition and translocation of human peroxisomal ABC transporter ALDP; Authors - Chao Xiong

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C