Anticorps Polyclonal de lapin anti-AADACL1

AADACL1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14021-1-AP

Synonymes

AADACL1, KIAA1363, KIAA1363, NCEH1,NCEH, NCEH, NCEH1

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Galerie de données de validation

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  WB analysis using 14021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14021-1-AP (AADACL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 14021-1-AP

  IP result of anti-AADACL1 (IP:14021-1-AP, 4ug; Detection:14021-1-AP 1:500) with COLO 320 cells lysate 1320 ug.

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  IHC staining of human brain using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of COLO 320 using 14021-1-AP

  Immunofluorescent analysis of COLO 320 cells using 14021-1-AP (AADACL1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3,
• Résultats positifs en IP: cellules COLO 320,
• Résultats positifs en IHC: tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules COLO 320

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

14021-1-AP cible AADACL1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AADACL1 Protéine recombinante Ag5136
• Nom complet: arylacetamide deacetylase-like 1
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC047588
• Symbole du gène: AADACL1
• Identification du gène (NCBI): 57552
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AADACL1(Arylacetamide deacetylase-like 1) is also named as NCEH1, KIAA1363 and belongs to the 'GDXG' lipolytic enzyme family. The transmembrane enzyme, AADACL1, controls the production of the monoalkylglycerol ether (MAGE) class of NELs in cancer cells and acts as a 2-acetyl MAGE hydrolase and is likely the principal source for this activity in tumor cells(PMID:21513884). The full length protein has three glycosylation sites and can be N-glycosylated(PMID:19159218). It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	WB	J Proteomics; Integrative proteomics-metabolomics strategy reveals the mechanism of hepatotoxicity induced by Fructus Psoraleae.; Authors - Jingyi Duan
human	IHC	J Transl Med; Multiplex immunohistochemistry defines two cholesterol metabolism patterns predicting immunotherapeutic outcomes in gastric cancer; Authors - Wei Tang
human	WB	Sci Rep; Lysophosphatidylglucoside/GPR55 signaling promotes foam cell formation in human M2c macrophages; Authors - Ryosuke Shimai