VWF Polyklonaler Antikörper

VWF Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Kat-Nr. : 27186-1-AP

Synonyme

F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, vwf

---

Galerie der Validierungsdaten

• 

  IHC staining of human tonsillitis using 27186-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27186-1-AP (VWF antibody) at dilution of 1:200 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with .

• 

  IHC staining of human tonsillitis using 27186-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27186-1-AP (VWF antibody) at dilution of 1:200 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with .

• 

  IHC staining of human breast cancer using 27186-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27186-1-AP (VWF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27186-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27186-1-AP (VWF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27186-1-AP (vwf antibody) at dilution of 1:200 (under 40x lens). proteolytic pre-treatment mediated antigen retrieved with .

• 

  IHC staining of mouse brain using 27186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27186-1-AP (vwf antibody) at dilution of 1:200 (under 10x lens). proteolytic pre-treatment mediated antigen retrieved with .

• 

  IHC staining of rat brain using 27186-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27186-1-AP (vwf antibody) at dilution of 1:200 (under 10x lens). Proteinase K was used for proteolytic pre-treatment mediated antigen retrieval.

• 

  IHC staining of rat brain using 27186-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27186-1-AP (vwf antibody) at dilution of 1:200 (under 40x lens). Proteinase K was used for proteolytic pre-treatment mediated antigen retrieval.

• 

  IHC staining of rat brain using 27186-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27186-1-AP (vwf antibody) at dilution of 1:200 (under 40x lens). Proteinase K was used for proteolytic pre-treatment mediated antigen retrieval.

• 

  IHC staining of rat brain using 27186-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27186-1-AP (vwf antibody) at dilution of 1:200 (under 10x lens). Proteinase K was used for proteolytic pre-treatment mediated antigen retrieval.

• 

  IF Staining of human tonsillitis using 27186-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 27186-1-AP (vwf antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 18 publications below

Produktinformation

27186-1-AP bindet in WB, IHC, IF, ELISA VWF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VWF fusion protein Ag25578
• Vollständiger Name: von Willebrand factor
• Gene symbol: VWF
• Gene ID (NCBI): 7450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. 

What is the molecular weight of VWF?

Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). 

What is the tissue specificity of VWF?

The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). 

What is the function of VWF?

The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155). 

What is the importance of post-translational modifications (PTMs) on VWF function?

During the synthesis of VWF, it must undergo extensive PTMs. The VWF monomers are subsequently N-glycosylated with the addition of a 12 N-linked high-mannose-containing oligosaccharide after the cleavage of the signal peptide in the endoplasmic reticulum. Dimerization of the pro-VWF follows glycosylation via carboxyl-termini disulphide bond formation. In the Golgi apparatus, the oligosaccharide chains are further modified into complex carbohydrates and multimerization of pro-VWF dimers occurs after another round of disulphide bond formation at the amino-termini end of the subunits (PMID: 6334089).

Publikationen

Species	Application	Title
rat	IF	Biomaterials; Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.; Authors - Xianzhen Dong
human	IF	Adv Healthc Mater; Platelet Membrane-Fused Circulating Extracellular Vesicles Protect the Heart from Ischemia/Reperfusion Injury; Authors - Jizong Jiang
mouse	IF	Acta Biomater; Selective inhibition of glycolysis in hepatic stellate cells and suppression of liver fibrogenesis with vitamin A-derivative decorated camptothecin micelles; Authors - Li Xiang
human	IF	Aging (Albany NY); Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies.; Authors - Xinjie Wu
rat	IF	J Cell Mol Med; Slight up-regulation of Kir2.1 channel promotes endothelial progenitor cells to transdifferentiate into a pericyte phenotype by Akt/mTOR/Snail pathway.; Authors - Xiaodong Cui
mouse	WB,IF	J Ethnopharmacol; Exploring the synergic mechanism of Ligusticum striatum DC. and borneol in attenuating BMECs injury and maintaining tight junctions against cerebral ischaemia based on the HIF-1α/VEGF signalling pathway; Authors - Xiaoxiong Song