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SARS-CoV-2 S protein (126-264 aa) Polyklonaler Antikörper

SARS-CoV-2 S protein (126-264 aa) Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus

Anwendung

ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28869-1-AP

Synonyme

SARS-CoV-2 Spike Glycoprotein (126-264 aa), spike, spike protein

Galerie der Validierungsdaten

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  • ELISA experiment of Recombinant protein using SARS-CoV-2 S protein (126-264 aa) Polyclonal antib (28869-1-AP)

    ELISA experiment of Recombinant protein using 28869-1-AP

    SARS-CoV-2 Spike Antibody (28869-1-AP) tested by ELISA.SARS-CoV-2 Spike protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (28869-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Geprüfte Anwendungen

Erfolgreiche Detektion in ELISARecombinant protein

Empfohlene Verdünnung

AnwendungVerdünnung
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

28869-1-AP bindet in ELISA SARS-CoV-2 S protein (126-264 aa) und zeigt Reaktivität mit Virus

Getestete Reaktivität Virus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SARS-CoV-2 S protein (126-264 aa) fusion protein Ag30679
Vollständiger Name SARS-CoV-2 Spike Protein
Berechnetes Molekulargewicht 141 kDa
GenBank-ZugangsnummerNC_045512
Gene symbol COVID-19 S Protein
Gene ID (NCBI) 43740568
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28869-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 126-264aa.