Phospho-Marcksl1 Polyklonaler Antikörper

Phospho-Marcksl1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (3)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10018-3-AP

Synonyme

MARCKS, MARCKS-like 1, PKCSL, Myristoylated alanine-rich C-kinase substrate, Marcksl1

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Galerie der Validierungsdaten

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  WB analysis using 10018-3-AP

  Non-treated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10018-3-AP (Phospho-Marcksl1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

10018-3-AP bindet in WB, ELISA Phospho-Marcksl1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myristoylated alanine rich protein kinase C substrate
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC046601
• Gene symbol: Marcks
• Gene ID (NCBI): 17118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. This antibody is raised against phospho-Marcksl1 (geneID:17357) with S93/S104 phosphorylated. Because of the sequence similarity, this antibody can recognize phospho-Marcksl1 and phospho-Marcks.

Publikationen

Species	Application	Title
bovine	WB	Proc Natl Acad Sci U S A; MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.; Authors - Jin Benjamin Y BY
rat	WB	J Neurosci; NADPH oxidase is required for the sensory plasticity of the carotid body by chronic intermittent hypoxia.; Authors - Peng Y-J YJ
rat	WB	J Physiol; 5-HT evokes sensory long-term facilitation of rodent carotid body via activation of NADPH oxidase.; Authors - Peng Ying-Jie YJ
rat	WB	Am J Physiol Cell Physiol; Involvement of PKCdelta and PKD in pulmonary microvascular endothelial cell hyperpermeability.; Authors - Tinsley John H JH
human	WB	Open Virol J; HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.; Authors - Fiala Milan M