pan-keratin Polyklonaler Antikörper

pan-keratin Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-26411

Synonyme

58 kDa cytokeratin, CK 5, CK5, Cytokeratin 5, K5, keratin 5, KRT5, KRT5A, pan-keratin, Type II keratin Kb5

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Galerie der Validierungsdaten

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  IF Staining of human colon cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

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  IF Staining of human colon cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

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  IF Staining of human lung cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200, CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

• 

  IF Staining of human lung cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200, CoraLite® Plus 488 CD3 antibody (CL488-60181, Clone: 3F3A1, green), CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

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  IF Staining of A431 using CL555-26411

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

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  IF Staining of A431 using CL555-26411

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

• 

  IF Staining of human lung cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

• 

  IF Staining of human lung cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

• 

  IF Staining of human breast cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

• 

  IF Staining of human breast cancer using CL555-26411

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®555 pan-keratin antibody (CL555-26411) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe, A431-Zellen, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-26411 bindet in IF pan-keratin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: pan-keratin fusion protein Ag24184
• Vollständiger Name: keratin 5
• Berechnetes Molekulargewicht: 590 aa, 62 kDa
• GenBank-Zugangsnummer: BC024292
• Gene symbol: KRT5
• Gene ID (NCBI): 3852
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.