Dynamitin (1-83 AA) Polyklonaler Antikörper

Dynamitin (1-83 AA) Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 10031-2-AP

Synonyme

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, p50 dynamitin, RBP50

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10031-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10031-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10031-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10031-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10031-2-AP

  IP result of anti-Dynamitin (1-83 AA) (IP:10031-2-AP, 3ug; Detection:10031-2-AP 1:800) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hodengewebe, Maushodengewebe, Mauslungengewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10031-2-AP bindet in WB, IP, ELISA Dynamitin (1-83 AA) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: dynactin 2 (p50)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC000718
• Gene symbol: Dynamitin
• Gene ID (NCBI): 10540
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 59aa of full length p50 dynamitin.

Publikationen

Species	Application	Title
mouse	WB	Nat Med; PMID: 19838201; Authors - Cheng Zhiyong Z

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

X (Verified Customer) (10-29-2020)

A very good p50 antibody to detect p50 at the righ MW by Western.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain/human brain lysates

Dynamitin (1-83 AA) Antibody Western Blot validation (1:2000 dilution) in mouse brain/human brain lysates (Cat no:10031-2-AP)