Cleaved Caspase 3 Polyklonaler Antikörper

Cleaved Caspase 3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (4)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(277)

Rezensionen (1)

Kat-Nr. : 25128-1-AP

Synonyme

CASP3, Caspase3, CASP 3, CASP-3, Caspase 3

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• 

  WB analysis using 25128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25128-1-AP (cleaved Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 25128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25128-1-AP (Cleaved Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25128-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cleaved Caspase 3 antibody (25128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 254 publications below
• IHC: See 30 publications below
• IF: See 21 publications below

Produktinformation

25128-1-AP bindet in WB, IHC, IF/ICC, ELISA Cleaved Caspase 3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: caspase 3, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 17-25 kDa
• GenBank-Zugangsnummer: NM_032991
• Gene symbol: Caspase 3
• Gene ID (NCBI): 836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize the cleaved Caspase 3 fragments. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved fragment of Caspase 3 might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

Publikationen

Species	Application	Title
human	WB,IHC	J Hazard Mater; Exposure to 6PPD-Q induces dysfunctions of ovarian granulosa cells: Its potential role in PCOS; Authors - Hanxi Yu
human	WB	Small; A Responsive Nanorobot Modulates Intracellular Zinc Homeostasis to Amplify Mitochondria-Targeted Phototherapy; Authors - Xinyuan Zhou
human	WB	Redox Biol; DNAJC12 causes breast cancer chemotherapy resistance by repressing doxorubicin-induced ferroptosis and apoptosis via activation of AKT; Authors - Mengjia Shen
mouse	WB	J Adv Res; TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway; Authors - Kai Sun
mouse	WB,IHC	Adv Healthc Mater; Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation; Authors - Ning Wang
chicken	WB,IF	Sci Total Environ; MitomiR-1736-3p regulates copper-induced mitochondrial pathway apoptosis by inhibiting AATF in chicken hepatocytes; Authors - Yuanxu Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vignesh (Verified Customer) (09-19-2025)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: 231cells