Chromogranin A Monoklonaler Antikörper

Chromogranin A Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2A7D11

Kat-Nr. : 60135-2-Ig

Synonyme

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

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Galerie der Validierungsdaten

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  WB analysis of mouse pancreas using 60135-2-Ig

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig pancreas using 60135-2-Ig

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig adrenal gland using 60135-2-Ig

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 60135-2-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 60135-2-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 60135-2-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 60135-2-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60135-2-Ig (CHGA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 60135-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60135-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60135-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60135-2-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60135-2-Ig (CHGA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas cancer using 60135-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human pancreas cancer using 60135-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of Neuro-2a using 60135-2-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed Neuro-2a cells using Chromogranin A antibody (60135-2-Ig, Clone: 2A7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, BxPC-3-Zellen, Hausschwein-Nebennierengewebe, Hausschwein-Pankreasgewebe, Neuro-2a-Zellen, PC-12-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Pankreaskarzinomgewebe, Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

60135-2-Ig bindet in WB, IHC, IF, ELISA Chromogranin A und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Chromogranin A fusion protein Ag0807
• Vollständiger Name: chromogranin A (parathyroid secretory protein 1)
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC006459
• Gene symbol: CHGA
• Gene ID (NCBI): 1113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Publikationen

Species	Application	Title
mouse	IF	Stem Cell Res Ther; Expansion of mouse castration-resistant intermediate prostate stem cells in vitro; Authors - Yalan Xu
human	IF	Front Pharmacol; Development of a small cell lung cancer organoid model to study cellular interactions and survival after chemotherapy; Authors - Chandani Sen
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang