cGAS Polyklonaler Antikörper

cGAS Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 29958-1-AP

Synonyme

cGAS, Cyclic GMP-AMP synthase, E330016A19Rik, Mb21d1, RIKEN cDNA E330016A19 gene

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Galerie der Validierungsdaten

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  WB analysis using 29958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29958-1-AP (cGAS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 29958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29958-1-AP (cGAS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 29958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 29958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 29958-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 29958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, A549-Zellen, C2C12-Zellen, HeLa-Zellen, MCF-7-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, THP-1-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

29958-1-AP bindet in WB, IHC, IF, ELISA cGAS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: cGAS fusion protein Ag31677
• Vollständiger Name: RIKEN cDNA E330016A19 gene
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC145651
• Gene symbol: E330016A19Rik
• Gene ID (NCBI): 214763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.

Publikationen

Species	Application	Title
mouse	IF	Mol Immunol; Inhibition of cGAS-STING pathway by stress granules after activation of M2 macrophages by human mesenchymal stem cells against drug induced liver injury; Authors - Yao Wang
mouse	WB	Eur J Pharmacol; Nitisinone attenuates cartilage degeneration and subchondral osteoclastogenesis in osteoarthritis and concomitantly inhibits the cGAS/STING/NF-κB pathway; Authors - Tao Yang