Beta Actin Monoklonaler Antikörper

Beta Actin Monoklonal Antikörper für IHC

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

Hausschwein, human, Maus, Pflanze, Ratte, Zebrafisch

Anwendung

WB, IHC

Konjugation

Biotin

Publikationen

4

CloneNo.

7D2C10

Kat-Nr. : Biotin-60008

Synonyme

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, PS1TP5BP1, β actin

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Galerie der Validierungsdaten

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  IHC staining of mouse heart using Biotin-60008

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-60008 (beta Actin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using Biotin-60008

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-60008 (beta Actin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

Biotin-60008 bindet in WB, IHC Beta Actin und zeigt Reaktivität mit Hausschwein, human, Maus, Pflanze, Ratten, Zebrafisch

• Getestete Reaktivität: Hausschwein, human, Maus, Pflanze, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Hausschwein, Ratte
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Beta Actin fusion protein Ag0297
• Vollständiger Name: actin, beta
• Berechnetes Molekulargewicht: 375 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC002409
• Gene symbol: ACTB
• Gene ID (NCBI): 60
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Publikationen

Species	Application	Title
human	WB	iScience; The vesicular transporter STX11 governs ATGL-mediated hepatic lipolysis and lipophagy.; Authors - Gaojian Zhang
pig	WB	Reproduction; FSH promotes immature porcine Sertoli cell proliferation by activating the CCR7/Ras-ERK signaling axis; Authors - Yanfei Yin
rat	WB	Exp Ther Med; Deciphering the role of TNF‑α‑induced protein 8‑like 2 in the pathogenesis of necrotizing enterocolitis in neonatal rats; Authors - Juan Luo
human	WB	Balkan Med J; Asiaticoside Down-Regulates HIF-1α to Inhibit Proliferation, Migration, and Angiogenesis in Thyroid Cancer Cells; Authors - Yi Zhang